The relative distribution and cellular localization of the dopamine D1 and D2 receptor
subtypes were assessed in frozen sections of rat medial prefrontal cortex (mPFC). The D1
and D2 receptor binding sites were labeled with the selective high-affinity antagonists SCH
23390 and N-(p-aminophenethyl)-spiperone (NAPS), respectively, coupled to either Bodipy
or Texas red fluorophores. Under the incubation conditions employed, kinetic, competition,
and selectivity studies showed that these modified ligands retained pharmacological …

In a recent study in rat medial prefrontal cortex (mPFC), a fluorescently coupled, high‐affinity
ligand for the D1 receptor subtype was localized to nonpyramidal neurons, while a ligand
selective for the D2 subtype was found on neurons with a size distribution overlapping with
both small pyramidal and large nonpyramidal cells. These observations raised the
possibility that a subpopulation of cortical neurons with an intermediate size range may
coexpress both the D1 and D2 receptor subtypes. In the present study, the D1 and D2 …