The present study investigated the changes in hepatic function indices in ulcerated rats pretreated with P. americana seed (PAM) and B. pinnatum leaf (BPI) ethyl acetate fraction. The study consisted of fifty (50) male wistar rats divided into 10 groups of five animals each as follows: The groups 1-5 were designated normal control (NC), ulcer control (UC), Omeprazole 20mgkg-1 (OMEP), PAM and BPI respectively. Binary mixtures of PAM and BPI were pretreated to groups 6-10 in the ratios PAM+ BPI (1: 1), PAM+ BPI (1: 2), PAM+ BPI (1: 3), PAM+ BPI (2: 1) and PAM+ BPI (3: 1) respectively. Rats in groups 4–10 were pre-treated for 21 days with 400 mgKg-1 body weight/day of single or binary mixtures of the fraction by intubation. Gastric ulcer was induced in rats of group 2-10 on 22nd day by administration of indomethacin (30mg/kg body weight) in a single dose. Results show that administration of PAM, PAM+ BPI (1: 1), and PAM+ BPI (3: 1) resulted in the elevation of AST and ALT activity; while ALT, AST activity, and globulin concentration were significantly (p< 0.05) reduced in OMEP, BPI, PAM+ BPI (1: 2) PAM+ BPI (2: 1), PAM+ BPI (1: 3) and UC, OMEP, PAM, PAM+ BPI (1: 2), PAM+ BPI (2: 1) and PAM+ BPI (3: 1) treated groups respectively. Also, serum albumin concentration and albumin/globulin ratio were increased in all groups exposed to indomethacin except for BPI and PAM+ BPI (1: 3) groups. The present study demonstrated that pre-treatment of the animals with binary combinations of PAM+ BPI (1: 1), PAM+ BPI (3: 1), and indomethacin may result in significant derangement of liver function and histopathological structures.