Characterization and Effects of the Replicated Flowering Time Gene FLC in Brassica rapa

ME Schranz, P Quijada, SB Sung, L Lukens… - Genetics, 2002 - academic.oup.com
ME Schranz, P Quijada, SB Sung, L Lukens, R Amasino, TC Osborn
Genetics, 2002academic.oup.com
Functional genetic redundancy is widespread in plants and could have an important impact
on phenotypic diversity if the multiple gene copies act in an additive or dosage-dependent
manner. We have cloned four Brassica rapa homologs (BrFLC) of the MADS-box flowering-
time regulator FLC, located at the top of chromosome 5 of Arabidopsis thaliana. Relative rate
tests revealed no evidence for differential rates of evolution and the ratios of
nonsynonymous-to-synonymous substitutions suggest BrFLC loci are not under strong …
Abstract
Functional genetic redundancy is widespread in plants and could have an important impact on phenotypic diversity if the multiple gene copies act in an additive or dosage-dependent manner. We have cloned four Brassica rapa homologs (BrFLC) of the MADS-box flowering-time regulator FLC, located at the top of chromosome 5 of Arabidopsis thaliana. Relative rate tests revealed no evidence for differential rates of evolution and the ratios of nonsynonymous-to-synonymous substitutions suggest BrFLC loci are not under strong purifying selection. BrFLC1, BrFLC2, and BrFLC3 map to genomic regions that are collinear with the top of At5, consistent with a polyploid origin. BrFLC5 maps near a junction of two collinear regions to Arabidopsis, one of which includes an FLC-like gene (AGL31). However, all BrFLC sequences are more closely related to FLC than to AGL31. BrFLC1, BrFLC2, and BrFLC5 cosegregate with flowering-time loci evaluated in populations derived by backcrossing late-flowering alleles from a biennial parent into an annual parent. Two loci segregating in a single backcross population affected flowering in a completely additive manner. Thus, replicated BrFLC genes appear to have a similar function and interact in an additive manner to modulate flowering time.
Oxford University Press
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