The aim of this study was to determine the relationship of antioxidants profile with endogenous reproductive hormone level across the menstrual cycle. Predicting ovulation is the basis on which the fertile period is determined. Currently there are many methods accessible to identify the ovulatory period. Unfortunately, these methods are not always efficient for accurate detection of ovulation. Hence, an attempt was made to detect ovulation through antioxidant profile with the help of BBT, saliva ferning and ultrasound. Salivary antioxidants were investigated serially during preovulatory, ovulatory and postovulatory periods of normal menstrual cycle in twenty healthy volunteers. The sample was collected in three consecutive menstrual cycles. Salivary antioxidants was estimated and analysis by UV-spectrophotometer. The results revealed higher significant variations in the antioxidant concentration and physiological changes are also essential owing to ovarian hormones during menstrual cycle. Alkaline phopshatase, peroxidase, ascorbic acid and lactate dehydrogenase were maximum during ovulation and minimum during postovulatory phase. Superoxide dismutase, catalase and glutathione peroxidase were decrease in prepubert and menopau cycle. Among these antioxidants alkaline phopshatase, peroxidase and ascorbic acid were predominantly exhibited during ovulatory phase than pre and post-ovulatory phase. It is concluded that of 17 â-oestradiol and progesterone infulence the antioxidative status and the vulnerability to oxidative stress exhibited during normal menstrual cycle would serve early diagnosis of ovulation detection.

At present time, there is no single, completely reliable parameter for ovulation prediction. Variations from the mean among hormonal and clinical parameters during a specific menstrual cycle are troublesome, and there is considerable variation from cycle to cycle, even in the same patient [1, 2]. The primary objective of this study is to develop a noninvasive and reliable method for ovulation detection. Saliva would be a suitable medium for this purpose. The sample collection process is noninvasive, painless and convenient. Collecting saliva samples is possible several times a day and can be conveniently accomplished under circumstances where it is difficult to get blood samples.[2].