Hardness is a kernel characteristic that influences both milling and processing characteristics of wheat. It is one characteristic that is used for segregating wheat to meet the needs for various products. Kernel virteousness is a visual marker for hardness and is the characteristic assessed during the grading process. The potential of classifying vitreous and non-vitreous durum wheat kernels, in crease-down position, using imaging systems based on real time soft X-rays or transmitted light was assessed in this study. Durum wheat kernels at 14, 15 and 16% moisture contents were used as samples in this study. Image features modeling gray level distribution, textural and shape moments were measured and used to develop a classification system for vitreous and non-vitreous durum wheat kernels. The classification accuracies were 76% for vitreous kernels and 82% for non-vitreous kernels at 16% moisture content using the soft X-ray system but for the transmitted light system, the classification accuracies were 86% for vitreous and 93% for non-vitreous kernels. Moisture content had no effect on classifying vitreous or non-vitreous kernels by the transmitted light system but the classification accuracies increased with moisture content for non-vitreous kernels by the soft X-ray system.