Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica)
S Borchetia, C Bora, B Gohain, P Bhagawati… - African Journal of …, 2011 - ajol.info
This report describes the cloning and expression of a gene lycopene epsilon
cyclase,(LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid
lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was
identified from an SSH library constructed for quality trait in tea. 5'and 3'RACE (rapid-
amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology
studies showed that the deduced amino acid sequence of LCYE gene had the highest …
cyclase,(LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid
lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was
identified from an SSH library constructed for quality trait in tea. 5'and 3'RACE (rapid-
amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology
studies showed that the deduced amino acid sequence of LCYE gene had the highest …
Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica).
SB Sangeeta Borchetia, CB Chiranjana Bora… - 2011 - cabidigitallibrary.org
This report describes the cloning and expression of a gene lycopene epsilon
cyclase,(LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid
lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was
identified from an SSH library constructed for quality trait in tea. 5′ and 3′ RACE (rapid-
amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology
studies showed that the deduced amino acid sequence of LCYE gene had the highest …
cyclase,(LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid
lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was
identified from an SSH library constructed for quality trait in tea. 5′ and 3′ RACE (rapid-
amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology
studies showed that the deduced amino acid sequence of LCYE gene had the highest …
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