Visualization of protein-protein interaction in nuclear and cytoplasmic fractions by co-immunoprecipitation and in situ proximity ligation assay

X Zhu, A Zelmer, S Wellmann - Journal of visualized …, 2017 - pmc.ncbi.nlm.nih.gov
assay detects protein-protein interactions with a unique technique involving rolling-circle
PCR, while conventional immunocytochemistry only shows co-localization of … binding affinity of …

Analysis of protein-protein interactions and co-localization between components of gap, tight, and adherens junctions in murine mammary glands

E Dianati, I Plante - Journal of visualized experiments: JoVE, 2017 - pmc.ncbi.nlm.nih.gov
co-localization of proteins by merging the images scanned with the lasers at the different
wavelengths. Note: Protein co-localization … -IF, this assay is based on antibody affinity. For this …

In situ proximity ligation assays indicate that hemochromatosis proteins Hfe and transferrin receptor 2 (Tfr2) do not interact

G Rishi, EM Crampton, DF Wallace… - PLoS One, 2013 - journals.plos.org
… In our co-expression system we observed nominal co-localization between Hfe and WT Tfr2
in punctuate intracellular vesicles (Panel 5 C and D). The minimal co-localization of the two …

Materials‐based strategies for multi‐enzyme immobilization and colocalization: a review

F Jia, B Narasimhan… - Biotechnology and …, 2014 - Wiley Online Library
… Originally such techniques were widely used in affinity chromatography for protein …
immunosorbent assay, streptavidin-tagged HRP can bind biotinylated antibody to amplify the assay

Co-localization of atmospheric H2 oxidation activity and high affinity H2-oxidizing bacteria in non-axenic soil and sterile soil amended with Streptomyces sp. PCB7

P Constant, SP Chowdhury, L Hesse… - Soil Biology and …, 2011 - Elsevier
assay of the hhyL gene was established to estimate the abundance of presumptive high affinity
… unveiled for the first time the co-localization of high affinity hydrogenase activity and H 2 -…

Co-localization of GABAA receptors and benzodiazepine receptors in the brain shown by monoclonal antibodies

P Schoch, JG Richards, P Häring, B Takacs, C Stähli… - Nature, 1985 - nature.com
… enhancement of benzodiazepine receptor affinity in micromolar concentrations of … assays
with "H-FNZ (10 nM, 60 min) and "H-muscimol (100 nM, 30 min). The radioligand binding assay

… -3 activation as an anti-cancer strategy: Structure− activity relationship of procaspase-activating compound 1 (PAC-1) and Its cellular co-localization with caspase-3

QP Peterson, DC Hsu, DR Goode… - Journal of medicinal …, 2009 - ACS Publications
assays provides a comprehensive data set used to formulate a structure−activity relationship
for PAC-1; brief descriptions of these assays … combination of moderate affinity for zinc while …

Definition of a novel ligand binding domain of a nuclear bHLH receptor: colocalization of ligand and hsp90 binding activities within the regulable inactivation domain …

ML Whitelaw, M Göttlicher, JA Gustafsson… - The EMBO …, 1993 - embopress.org
… an excess of the high affinity dioxin receptor ligand, 2,3,7,8-tetrachlorodibenzofuran … affinity
glucocorticoid receptor ligand, dexamethasone (Figure 3). In conclusion, these in vitro assays

New insights into the DT40 B cell receptor cluster using a proteomic proximity labeling assay

XW Li, JS Rees, P Xue, H Zhang, SW Hamaia… - Journal of Biological …, 2014 - ASBMB
… The labeled proteins can then be isolated by standard affinity chromatography, and … For
co-localization of chB6 and lipid rafts, ∼10 6 cells were suspended with primary antibody FU5-…

Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay

O Söderberg, KJ Leuchowius, M Gullberg, M Jarvius… - Methods, 2008 - Elsevier
… Triple binder PLA reactions can also reveal the co-localization of sets of three proteins in a
affinity for each other using this method do interact under normal conditions, and the assay