… ) to analyze potD mRNA expression under physiologically … PsaA, a lipoprotein component
of an Mn +2 ABC transport … tubes and incubated in 5% CO 2 at 37 C. Bacteria were grown in …

… 5% CO 2 using a semi-synthetic casein hydrolysate medium supplemented with yeast extract
(C + Y medium; concentration of Mn 2+ … failed, therefore we used inverse PCR to obtain the …

… Streptococcus pneumoniae er en bakterie … expression of the gene behind PcomX. Another
system that is widely used is the Zn2+-system where you can regulate the gene expression …

… A new reverse primer with an XhoI site (Table 2) was … broth and grown at 37C with 5% CO
2 . The growth of each strain was … Our results showed that both proteins preferred Mn 2+ from …

… gene expression in Streptococcus pneumoniae. In this study, expression of key pneumococcal
virulence genes … In all cases, a PCR product was only seen in the presence of reverse …

… Replacement of Zn 2+ with Co 2+ results … protein concentration. CD spectra were recorded
for apoZnuA alone and in the presence of various metal ions (Zn 2+ , Mn 2+ , Cd 2+ , and Co 2…

… donate a proton to the opposite side of the stabilized enolate … Protein expression was
induced when the bacterial cul ture … was reported to be inhibited by Mn2+, Cu2+, and Zn2+ [23]. …

… , we performed reverse transcription polymerase chain … an important global modulator of
gene expression, which is required … Mn 2+ with other divalent metal cations (Mg 2+ , Co 2+ , Ni 2…

… 37C and 5% CO 2 . The … genes responsible for such a phenotype, gene expression studies
for oxidative stress response in the presence of polyamines was performed. The expression …

… of the map gene product from Streptococcus pneumoniae, S. … of the DNA fragment obtained
by inverse PCR. The DNA was … the addition of Co 2+ and, to a less extent, by Mn 2+ but not …