Colorimetric protein quantification in aqueous two-phase systems
M González-González, K Mayolo-Deloisa… - Process …, 2011 - Elsevier
The polymer-salt rich environment of aqueous two-phase systems disturbs standard protein
quantification methods, like UV measurement or Bradford assay. Therefore, we
systematically investigated the influence of high polyethylene glycol and phosphate salt
concentrations on the readings of three colorimetric protein assays: Bradford, DC (Bio-Rad)
and ninhydrin assay. All three assays are troubled by the aqueous two-phase systems
components, but disturbances can be significantly reduced by diluting the samples to 1 …
quantification methods, like UV measurement or Bradford assay. Therefore, we
systematically investigated the influence of high polyethylene glycol and phosphate salt
concentrations on the readings of three colorimetric protein assays: Bradford, DC (Bio-Rad)
and ninhydrin assay. All three assays are troubled by the aqueous two-phase systems
components, but disturbances can be significantly reduced by diluting the samples to 1 …