To investigate whether combination of vitamin C and E able to inhibit decreasing angiogenesis, endometrial thickness, and α-estrogen receptor level in female rats receiving orally MSG-treatment.

Twenty five female Wistar rats were divided into five group, control group, MSG [140 mg/200 gram body weight (bw)] group non treated and treated with combined vitamin C (0.2; 0.4; or 0.8 mg/g bw) and E (0.04 IU/g bw). Analysis of vascular endothelial growth factor (VEGF) level were done by immunohistochemistry technique. Analysis of the number of arteriole and thickness of endometrium was done histopathologically with hematoxylin eosin staining. Analysis of uterus α-estrogen receptor was done using flowcytometer.

The expression of VEGF, number of arteriole, thickness of endometrium, and α-estrogen receptor were significantly lower in MSG-treatment group compared to control group (P < 0.05). Second and third dose of combined vitamin C and E significantly increased VEGF level and number of arteriole compared to MSG-treatment group (P < 0.05), to reach similar level in control group (P > 0.05). Administration of vitamin C and E significanlty increased the thickness of endometrium, and expression of α-estrogen receptor compared to MSG-treatment group (P < 0.05), reaching the number in the control group (P > 0.05).

The present data suggesting that combined vitamin C and E able to inhibit endometrial toxicity caused by orally MSG treatment via modulating angiogenesis, increase endometrial thickness and expression of α-estrogen receptor.