The increasing incidence of cancer coupled with clinical failure of orthodox medicines and rising costs of irradiation and surgical interventions that impact negatively on access to care particularly in low and middle income countries including Nigeria have become a serious global health problem. Hence an edible mushroom, Schizophyllum commune was explored in this study, for the discovery, development and delivery of novel anticancer agents. S. commune wild type and mutant strains generated by exposing the wild type organism to Ultraviolet rays at various time intervals of 30, 60 and 90 minutes coded as SCW, SCM1, SCM2 and SCM3 respectively were grown in submerged liquid medium containing Hibiscus sabdariffa calyx extract enriched with yeast extract and peptone water under continuous aeration for six days. Thereafter Gas Chromatography-Mass Spectrophotometric and Cytotoxicity assay using Hep2 cells were carried out on the broth. The GC-MS inferred over hundred diverse organic compounds present in all the