Roses are an important commercial cut flower with a wide range of varieties throughout the world. Due to its economic value, this study aimed to optimize the in vitro propagation protocol of three rose cultivars ie two (Kashan and Kazanlik) from Rosa damascene Mill. and one (Tuscany superb) from R. gallica by comparing the micro-propagation stages of the musing nodal explants. Using nano-silver in the medium with concentrations of 0, 25, 50 and 75 ĩmol in a completely randomized design. Result showed that the quality of Kashan cultivar explant increased while it had adverse effect on the other cultivars. The results obviously showed that liquid medium containing 1.5 mg1-1 6-benzylaminopurine (BAP) affected proliferation depending on rose cultivar. Modified Murashige and Skoog (1962)(MS) medium fortified with 2.0 mg l-1 (BAP) and 0.2 mg1-1 indole-3-butyric acid (IBA) gave optimal shoot proliferation. Micro shoots from BAP induced cultures could be rooted easily on indole-3-butyric acid (IBA) supplemented medium. Treatment with IBA (2 mg1-1) was very effective for root induction. Effect of plant growth regulators on proliferation and rooting was also different depending on cultivar. The rooted plants were successfully transferred to pots after 15 d of hardening in a mist chamber. The present work highlights the significance of rose species and cultivars difference during micro propagation.