Comparison of ELISA, nested PCR and sequencing and a novel qPCR for detection of Giardia isolates from Jordan
Little is known about the prevalence of Giardia duodenalis in human patients in Jordan and
all previous studies have used direct microscopy, which lacks sensitivity. The present study
developed a novel quantitative PCR (qPCR) assay at the β-giardin (bg) locus and evaluated
its use as a frontline test for the diagnosis of giardiasis in comparison with a commercially
available ELISA using nested PCR and sequencing of the glutamate dehydrogenase (gdh)
locus (gdh nPCR) as the gold standard. A total of 96 human faecal samples were collected …
all previous studies have used direct microscopy, which lacks sensitivity. The present study
developed a novel quantitative PCR (qPCR) assay at the β-giardin (bg) locus and evaluated
its use as a frontline test for the diagnosis of giardiasis in comparison with a commercially
available ELISA using nested PCR and sequencing of the glutamate dehydrogenase (gdh)
locus (gdh nPCR) as the gold standard. A total of 96 human faecal samples were collected …
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