Visceral leishmaniasis (VL), also known as kala-azar, is a disseminated protozoan infection
caused by Leishmania donovani complex. Traditionally the definite diagnosis is made by
amastigote detection in the tissue. The aim this study was to evaluate the PCR technique in
stained slides of bone marrow and lymph nodes aspirates with suspect diagnosis for
leishmaniasis. Slides were selected totaling 62 suspect cases (33 bone marrow samples
and 29 lymph node samples) and 17 positive cases (8 bone marrow and 9 lymph node) …