Comparison of detection methods for banana bract mosaic virus in banana (Musa sp.).
MK Dhanya, B Rajagopalan, K Umamaheswaran… - 2007 - cabidigitallibrary.org
MK Dhanya, B Rajagopalan, K Umamaheswaran, R Ayisha
2007•cabidigitallibrary.orgBanana bract mosaic virus (BBrMV) locally known as Kokkan causes serious threat to
banana production and it spreads at a high rate in almost all the banana varieties cultivated
in Kerala, India. Serological techniques ie chloroplast agglutination, microprecipitin test,
agar gel double diffusion test, direct antigen coating-enzyme linked immunosorbant assay
(DAC-ELISA) and dot immunobinding assay (DIBA) were compared to evaluate the
performance of the diagnosis of the virus. Each method was conducted using extract of …
banana production and it spreads at a high rate in almost all the banana varieties cultivated
in Kerala, India. Serological techniques ie chloroplast agglutination, microprecipitin test,
agar gel double diffusion test, direct antigen coating-enzyme linked immunosorbant assay
(DAC-ELISA) and dot immunobinding assay (DIBA) were compared to evaluate the
performance of the diagnosis of the virus. Each method was conducted using extract of …
Abstract
Banana bract mosaic virus (BBrMV) locally known as Kokkan causes serious threat to banana production and it spreads at a high rate in almost all the banana varieties cultivated in Kerala, India. Serological techniques i.e. chloroplast agglutination, microprecipitin test, agar gel double diffusion test, direct antigen coating-enzyme linked immunosorbant assay (DAC-ELISA) and dot immunobinding assay (DIBA) were compared to evaluate the performance of the diagnosis of the virus. Each method was conducted using extract of infected tissues and antiserum developed against the virus. ELISA and DIBA were more sensitive compared to the other methods tested. DAC-ELISA was the most economical method for the detection of BBrMV.
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