The aim of this study was to compare the prevalence of selected pathogens in ticks taken from cats and dogs and from vegetation in urban settlements. A study was conducted to estimate the distribution of Anaplasma phagocytophilum and Borrelia burgdorferi in adult Ixodes ricinus (236), Dermacentor reticulatus (237) and Ixodes hexagonus (3) ticks collected from animals in veterinary clinics (184) and from vegetation in urban settlements (292). The most numerous ticks collected from animals were Ixodes ricinus (73.9%), followed by Dermacentor reticulatus (24.5%) and Ixodes hexagonus (1.6%). A total of 65.8% of the ticks collected from vegetation were Dermacentor reticulatus and 30% were Ixodes ricinus. The arthropods removed from the animals were most commonly located around the neck (48.1%) and in the mouth area (17.1%). All ticks were analyzed by molecular techniques. The percentages of ticks positive for Borrelia burgdorferi and Anaplasma phagocytophilum among those collected from animals differed from the corresponding rates for ticks taken from vegetation in the same area. Anaplasma phagocytophilum was more common in ticks collected from vegetation (N= 137, or 47.20%) than in those from animals (N= 12, or 6.6%). Borrelia burgdorferi, as well, was more common in ticks collected from the vegetation (N= 96, or 32.9%) than in those from animals (N= 19, or 10.5%). The DNA of A. phagocytophilum and B. burgdorferi were detected in 30.4% and 22.8% of D. reticulatus ticks, respectively, and in 32.6% and 25.4% of I. ricinus ticks, respectively. The DNA of A. phagocytophilum was also found in one Ixodes hexagonus tick. Single infections were noted in 69 I. ricinus ticks, 56 D. reticulatus ticks and 1 I. hexagonus tick. Coinfections of A. phagocytophilum with B. burgdorferi were detected in 33 (14.0%) I. ricinus ticks and in 29 (12.24%) D. reticulatus ticks. Infected companion animals can form a reservoir for human tick-transmitted infectious agents. The monitoring of the pathogens transmitted by ticks is an important tool in preventing and combating infections transmitted to humans and animals.