Comparison of the performance of ITS1 and ITS2 as barcodes in amplicon-based sequencing of bioaerosols
This paper presents the performance of two eukaryotic genomic ribosomal regions, ITS1 and
ITS2, in describing fungal diversity in aerosol samples using amplicon-based High-
Throughput Sequencing (HTS). Composting sites, biomethanization facilities, and dairy
farms, all affected by the presence of fungi, were visited to collect air samples. The amplicon-
based HTS approach is a target enrichment method that relies on the amplification of a
specific target using particular primers before sequencing. Thus, the results are highly …
ITS2, in describing fungal diversity in aerosol samples using amplicon-based High-
Throughput Sequencing (HTS). Composting sites, biomethanization facilities, and dairy
farms, all affected by the presence of fungi, were visited to collect air samples. The amplicon-
based HTS approach is a target enrichment method that relies on the amplification of a
specific target using particular primers before sequencing. Thus, the results are highly …
[引用][C] Comparison of the performance of ITS1 and ITS2 as barcodes in amplicon-based sequencing of bioaerosols. PeerJ 8: e8523
H Mbareche, M Veillette, G Bilodeau, C Duchaine - 2020