Compensation of missing wedge effects with sequential statistical reconstruction in electron tomography
Electron tomography (ET) of biological samples is used to study the organization and the
structure of the whole cell and subcellular complexes in great detail. However, projections
cannot be acquired over full tilt angle range with biological samples in electron microscopy.
ET image reconstruction can be considered an ill-posed problem because of this missing
information. This results in artifacts, seen as the loss of three-dimensional (3D) resolution in
the reconstructed images. The goal of this study was to achieve isotropic resolution with a …
structure of the whole cell and subcellular complexes in great detail. However, projections
cannot be acquired over full tilt angle range with biological samples in electron microscopy.
ET image reconstruction can be considered an ill-posed problem because of this missing
information. This results in artifacts, seen as the loss of three-dimensional (3D) resolution in
the reconstructed images. The goal of this study was to achieve isotropic resolution with a …
[PDF][PDF] Compensation of Missing Wedge Effects with Sequential Statistical Reconstruction in
L Paavolainen, E Acar, U Tuna, S Peltonen, T Moriya - 2014 - academia.edu
Electron tomography (ET) of biological samples is used to study the organization and the
structure of the whole cell and subcellular complexes in great detail. However, projections
cannot be acquired over full tilt angle range with biological samples in electron microscopy.
ET image reconstruction can be considered an ill-posed problem because of this missing
information. This results in artifacts, seen as the loss of three-dimensional (3D) resolution in
the reconstructed images. The goal of this study was to achieve isotropic resolution with a …
structure of the whole cell and subcellular complexes in great detail. However, projections
cannot be acquired over full tilt angle range with biological samples in electron microscopy.
ET image reconstruction can be considered an ill-posed problem because of this missing
information. This results in artifacts, seen as the loss of three-dimensional (3D) resolution in
the reconstructed images. The goal of this study was to achieve isotropic resolution with a …
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