In this study, we present a simple and economical method that enables rapid quantification
of amino acids based on their polymerization into a signal-generating protein. This method
harnesses amino acid-deficient cell-free protein synthesis systems that generate
fluorescence signals in response to exogenous amino acids. When premixed with assay
samples containing the amino acids in question, incubation of the cell-free synthesis
reaction mixture rapidly resulted in the production of sfGFP, the fluorescence intensity of …

In this study, we present a simple and economical method that enables rapid quantification
of amino acids based on their polymerization into a fluorescence protein. In this method that
harnesses an amino acid-deficient cellfree synthesis system as a conditional signal-
generating module, reporter proteins are generated when the reaction mixture is supplied
with specific amino acids [1]. When premixed with assay samples containing the amino
acids in question, incubation of the cell-free synthesis reaction mixture rapidly resulted in the …