Component co-expression and purification of recombinant human pyruvate dehydrogenase complex from baculovirus infected SF9 cells
Y Jiang, J Wang, G Zhang, K Oza, L Myers… - Protein Expression and …, 2014 - Elsevier
The mammalian pyruvate dehydrogenase complex (PDC) is a multi-component
mitochondrial enzyme that plays a key role in the conversion of pyruvate to acetyl-CoA
connecting glycolysis to the citric acid cycle. Recent studies indicate that targeting the
regulation of PDC enzymatic activity might offer therapeutic opportunities by inhibiting
cancer cell metabolism. To facilitate drug discovery in this area, a well defined PDC sample
is needed. Here, we report a new method of producing functional, recombinant, high quality …
mitochondrial enzyme that plays a key role in the conversion of pyruvate to acetyl-CoA
connecting glycolysis to the citric acid cycle. Recent studies indicate that targeting the
regulation of PDC enzymatic activity might offer therapeutic opportunities by inhibiting
cancer cell metabolism. To facilitate drug discovery in this area, a well defined PDC sample
is needed. Here, we report a new method of producing functional, recombinant, high quality …
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