Composite of Chitosan-Collagen-Aloe vera for Scaffolds Application on Skin Tissue

A Isfandiary, P Widiyanti… - Journal of Biomimetics …, 2017 - Trans Tech Publ
A Isfandiary, P Widiyanti, D Hikmawati
Journal of Biomimetics, Biomaterials and Biomedical Engineering, 2017Trans Tech Publ
In case of burns, scaffolds serve as cover for burns and facilitate cells regeneration.
Accordingly, this study seeks procedures to synthesize composite scaffolds for burns
utilizing collagen-chitosan-Aloe vera to find the best concentration of A loe vera as scaffolds
application. The synthesis of scaffolds was performed with collagen constituent composition
as follow: chitosan (1: 1) was dissolved in 0.05 M Acetic Acid, then subsequent variations of
Aloe vera (AV), namely 0% AV; 0.1% AV; 0.15% AV; 0.2% AV; and 0.25% AV were added …
In case of burns, scaffolds serve as cover for burns and facilitate cells regeneration. Accordingly, this study seeks procedures to synthesize composite scaffolds for burns utilizing collagen-chitosan-Aloe vera to find the best concentration of Aloe vera as scaffolds application. The synthesis of scaffolds was performed with collagen constituent composition as follow: chitosan (1:1) was dissolved in 0.05M Acetic Acid, then subsequent variations of Aloe vera (AV), namely 0% AV; 0.1% AV; 0.15% AV; 0.2% AV; and 0.25% AV were added into collagen-chitosan solution, and freeze dry method was applied. Sample characterization was done by FTIR, tensile strength test, SEM test, cytotoxicity assay and degradation test. Typical absorption bands of collagen in the FTIR test was obtained at 1645.5 cm-1 (C = O stretch), chitosan at 1540.28 cm-1 (NH stretch) and Aloe vera at 3474.41 cm-1 (NH stretch). Tensile test data showed the highest number of tensile strength from the 0.1% AV sample at 0.017 MPa. The SEM test revealed a pore size of <50 μm. During cytotoxicity assay, the entire sample is not toxic. Degradation test demonstrated that overall sample was not exhausted within two weeks. In conclusion, sample with 0.2% AV was potential as scaffolds for burns skin tissue.
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