Construction of Plasmid Vector for Expression of Bacteriocin N15-Encoding Gene and Effect of Engineered Bacteria on Enterococcus faecalis
M Lertcanawanichakul - Current microbiology, 2007 - Springer
Abstract A 6.09-kb plasmids vector pOri253 was constructed from the plasmid pIL253 (5.2
kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid
pOri253 conferred erythromycin resistance in both Escherichia coli and Enterococcus
faecalis. It has unique sites for Eco RI, Bam HI, Sal I, and Pst I derived from pIL253 and was
lost at a low rate in E. faecalis JCM8726 when cultured in Man, Rogosa, & Sharpe broth
without antibiotic. The lactococcal promoter P23 was inserted at one end of the pOri253 …
kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid
pOri253 conferred erythromycin resistance in both Escherichia coli and Enterococcus
faecalis. It has unique sites for Eco RI, Bam HI, Sal I, and Pst I derived from pIL253 and was
lost at a low rate in E. faecalis JCM8726 when cultured in Man, Rogosa, & Sharpe broth
without antibiotic. The lactococcal promoter P23 was inserted at one end of the pOri253 …
Construction of a Plasmid Vector for Expression of Bacteriocin N15-encoding Gene and Effect of Engineered Bacteria on Enterococcus Faecalis
M LERTCANAWANICHAKUL - Walailak Journal of Science and …, 2005 - wjst.wu.ac.th
Abstract A 6.09-kb plasmids vector pOri253 was constructed from the plasmid pIL253 (5.2
kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid
pOri253 conferred erythromycinresistance in both Escherichia coli and Enterococcus
faecalis. It has a unique site for each of EcoRI, BamHI, SalI and PstI derived from pIL253.
The plasmid is quite stable in E. faecalis JCM8726 when cultured in Mann Rogosa Sharpe
broth without antibiotic. The lactococcal promoter P23 was inserted at one end of the …
kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid
pOri253 conferred erythromycinresistance in both Escherichia coli and Enterococcus
faecalis. It has a unique site for each of EcoRI, BamHI, SalI and PstI derived from pIL253.
The plasmid is quite stable in E. faecalis JCM8726 when cultured in Mann Rogosa Sharpe
broth without antibiotic. The lactococcal promoter P23 was inserted at one end of the …
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