Acacia mangium is the main plantation species in Vietnam, accounting for more than 54% of the plantation forest estate. However, acacia plantations throughout Vietnam have been widely infected by Ceratocystis manginecans wilt disease, posing a serious threat to the long-term commercial viability of the species. Experiments were performed for the control of C. manginecans on Acacia mangium, testing nine different chemical fungicides, three biological agents, and four bacterial endophytes isolated from Acacia mangium. Experiments in vitro showed the fungicides Lanomyl 680WP, Ao’yo 300SC, Carbenzim 500FL and Ridomil Golf 68WG have a strong inhibitory effect against C. manginecans. The biological agent Biobus 100WP and one bacterial endophyte strain (Bacillus tequilensis K7) isolated from Acacia mangium also had strong inhibitory activity. These fungicides, biological agents and endophytes could also inhibit C. manginecans growth on excised leaves and one-year-old seedlings. These fungicides, biological agents and bacterial endophytes show potential for the control of C. manginecans wilt disease on acacia plantations, although further work is required to determine their efficacy in field trials.