[HTML][HTML] Controlled bacteriophage release from poly (ethylene glycol) hydrogels significantly reduces infection in a bone implant-associated infection model
Front. Bioeng. Biotechnol. Conference 10th World Biomaterials Congress, 2016•frontiersin.org
Materials and Methods: PEG-mal hydrogel synthesis: PEG-maleimide hydrogels [3] were
functionalized with the collagen mimetic peptide GFOGER and cross-linked with protease
degradable peptides. Bacteriophage release: A bacteriophage mixture of ɸPaer4, ɸPaer14,
ɸPaer22, and ɸW2005A was labeled with AlexaFluor 488 and encapsulated in PEG
hydrogels cross-linked with VPM, GPQ-W, or PEG-dithiol. Hydrogels were swollen in DPBS
or 2U/mL collagenase and fluorescence of the swelling supernatant was measured.
functionalized with the collagen mimetic peptide GFOGER and cross-linked with protease
degradable peptides. Bacteriophage release: A bacteriophage mixture of ɸPaer4, ɸPaer14,
ɸPaer22, and ɸW2005A was labeled with AlexaFluor 488 and encapsulated in PEG
hydrogels cross-linked with VPM, GPQ-W, or PEG-dithiol. Hydrogels were swollen in DPBS
or 2U/mL collagenase and fluorescence of the swelling supernatant was measured.
Materials and Methods: PEG-mal hydrogel synthesis: PEG-maleimide hydrogels [3] were functionalized with the collagen mimetic peptide GFOGER and cross-linked with protease degradable peptides.
Bacteriophage release: A bacteriophage mixture of ɸPaer4, ɸPaer14, ɸPaer22, and ɸW2005A was labeled with AlexaFluor 488 and encapsulated in PEG hydrogels cross-linked with VPM, GPQ-W, or PEG-dithiol. Hydrogels were swollen in DPBS or 2U/mL collagenase and fluorescence of the swelling supernatant was measured.
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