Ligand binding to the TCR causes a conformational change at the CD3 subunits to expose
the CD3ε cytoplasmic proline-rich sequence (PRS). It was suggested that the PRS is
important for TCR signaling and T cell activation. It has been shown that the purified,
recombinant SH3. 1 domain of the adaptor molecule noncatalytic region of tyrosine kinase
(Nck) can bind to the exposed PRS of CD3ε, but the molecular mechanism of how full-length
Nck binds to the TCR in cells has not been investigated so far. Using the in situ proximity …

Paensuwan, P., FA Hartl, OS Yousefi, J. Ngoenkam, P. Wipa, E. Beck-Garcia, EP Dopfer, B.
Khamsri, D. Sanguansermsri, S. Minguet, WW Schamel, and S. Pongcharoen. 2016. Nck
binds to the T cell antigen receptor using its SH3. 1 and SH2 domains in a cooperative
manner, promoting TCR functioning. J. Immunol. 196: 448–458.A source of funding was
omitted in this article as originally published. The corrected funding information footnote
should read:“PP and PW were supported by the Royal Golden Jubilee Ph. D. Programme of …