Marine bacterial and archaeal communities control global biogeochemical cycles through
nutrient acquisition processes that are ultimately dictated by the metabolic requirements of
individual cells. Currently lacking, however, is a sensitive, quick, and quantitative
measurement of activity in these single cells. We tested the applicability of copper (I)-
catalyzed cycloaddition, or “click,” chemistry to observe and estimate single-cell protein
synthesis activity in natural assemblages and isolates of heterotrophic marine bacteria …

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