Cpf1 enables fast and efficient genome editing in Aspergilli
KG Vanegas, ZD Jarczynska, T Strucko… - Fungal Biology and …, 2019 - Springer
Background CRISPR technology has revolutionized fungal genetic engineering by
increasing the speed and complexity of the experiments that can be performed. Moreover,
the efficiency of the system often allows genetic engineering to be introduced in non-model
species. The efficiency of CRISPR gene editing is due to the formation of specific DNA
double-strand breaks made by RNA guided nucleases. In filamentous fungi, only Cas9 has
so far been used as the CRISPR nuclease. Since, gene editing with Cas9 is limited by its …
increasing the speed and complexity of the experiments that can be performed. Moreover,
the efficiency of the system often allows genetic engineering to be introduced in non-model
species. The efficiency of CRISPR gene editing is due to the formation of specific DNA
double-strand breaks made by RNA guided nucleases. In filamentous fungi, only Cas9 has
so far been used as the CRISPR nuclease. Since, gene editing with Cas9 is limited by its …
[引用][C] Cpf1 enables fast and efficient genome editing in Aspergilli. Fungal Biol Biotechnol. 2019; 6: 6
KG Vanegas, ZD Jarczynska, T Strucko, UH Mortensen
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