Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa: effects of egg yolk, glycerol and cooling rate
MR Fernández-Santos, MC Esteso, V Montoro… - Theriogenology, 2006 - Elsevier
Two experiments were conducted to determine the effects of egg yolk (EY), glycerol, and
cooling rate on the cryosurvival of red deer epididymal spermatozoa. The aim of Experiment
1 was to examine the effects of two EY types (clarified EY, CE, prepared by centrifugation,
and whole EY, WE), and four EY concentrations (0, 5, 10 and 20%) on cryosurvival of red
deer epididymal spermatozoa. Sperm samples were diluted to a final sperm concentration
of∼ 200× 106spermatozoa/ml with a Tris-citrate-fructose-EY extender (TCF) prior to …
cooling rate on the cryosurvival of red deer epididymal spermatozoa. The aim of Experiment
1 was to examine the effects of two EY types (clarified EY, CE, prepared by centrifugation,
and whole EY, WE), and four EY concentrations (0, 5, 10 and 20%) on cryosurvival of red
deer epididymal spermatozoa. Sperm samples were diluted to a final sperm concentration
of∼ 200× 106spermatozoa/ml with a Tris-citrate-fructose-EY extender (TCF) prior to …
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