Cultivation-free typing of bacteria using optical DNA mapping
A variety of pathogenic bacteria can infect humans, and rapid species identification is crucial
for the correct treatment. However, the identification process can often be time-consuming
and depend on the cultivation of the bacterial pathogen (s). Here, we present a stand-alone,
enzyme-free, optical DNA mapping assay capable of species identification by matching the
intensity profiles of large DNA molecules to a database of fully assembled bacterial
genomes (> 10 000). The assay includes a new data analysis strategy as well as a general …
for the correct treatment. However, the identification process can often be time-consuming
and depend on the cultivation of the bacterial pathogen (s). Here, we present a stand-alone,
enzyme-free, optical DNA mapping assay capable of species identification by matching the
intensity profiles of large DNA molecules to a database of fully assembled bacterial
genomes (> 10 000). The assay includes a new data analysis strategy as well as a general …