In order for a pathologist to perform histological examination of a tissue sample, the sample
typically must first undergo formalin fixation and paraffin embedding (FFPE) 1. Formalin
fixation of the tissue ensures the tissue structure and morphology remain intact during
storage and processing and supports subsequent sectioning of the tissue. Thin tissue
sections (usually about 4-6 μm) are mounted on microscope slides and stained with dyes
that generate optical contrast for distinguishing cell nuclei, cytoplasmic components …