An experiment was designed to determine the synergistic effect of cold temperature and cycloheximide (CHI) on the postharvest performance of cut sprays of Dianthus chinensis. Sprays were harvested at 800 h with the oldest bud at paint brush stage, cut to a length of 27 cm and wet stored at 5 °C for 72 h before or after 1 h 0.1 mM CHI pulse. Another set of sprays was pulsed with 0.1 mM CHI for 1 h during storage (after 35 ½ h). A set of unpulsed sprays stored at 5 °C represented control. The treatment effects were evaluated by placing the sprays in holding solution comprising of distilled water and sucrose (SUC) (0.05 M) at room temperature (21 ± 2 °C) in the laboratory. The sprays wet stored at 5 °C and pulse treated with 0.1 mM CHI after 72 h wet storage showed an increment of vase life by 3 days; besides sustained rate of blooming, higher fresh and dry mass, floral diameter and sugar fractions, but maintained lower soluble protein content in comparison to the control. Vase life, fresh mass, dry mass and floral diameter of sprays wet stored at 5 °C and 1 h pulse treated with 0.1 mM CHI before and during storage was considerably lower than the sprays 1 h pulse treated with 0.1 mM CHI after storage, but significantly higher than that of the control. The study revealed that 1 h pulse treatment with 0.1 mM CHI after 72 h wet storage at 5 °C, followed by transfer to SUC can be used as an effective postharvest storage protocol to bring about the transportation of this beautiful cut flower within 72 h.