Cytoplasmic Nucleation and Atypical Branching Nucleation Generate Endoplasmic Microtubules in Physcomitrella patens
The mechanism underlying microtubule (MT) generation in plants has been primarily
studied using the cortical MT array, in which fixed-angled branching nucleation and katanin-
dependent MT severing predominate. However, little is known about MT generation in the
endoplasm. Here, we explored the mechanism of endoplasmic MT generation in protonemal
cells of Physcomitrella patens. We developed an assay that utilizes flow cell and oblique
illumination fluorescence microscopy, which allowed visualization and quantification of …
studied using the cortical MT array, in which fixed-angled branching nucleation and katanin-
dependent MT severing predominate. However, little is known about MT generation in the
endoplasm. Here, we explored the mechanism of endoplasmic MT generation in protonemal
cells of Physcomitrella patens. We developed an assay that utilizes flow cell and oblique
illumination fluorescence microscopy, which allowed visualization and quantification of …
[PDF][PDF] Cytoplasmic Nucleation and Atypical Branching Nucleation Generate Endoplasmic Microtubules in
Y Nakaoka, A Kimura, T Tani, G Goshimaa - scholar.archive.org
The mechanism underlying microtubule (MT) generation in plants has been primarily
studied using the cortical MT array, in which fixed-angled branching nucleation and katanin-
dependent MT severing predominate. However, little is known about MT generation in the
endoplasm. Here, we explored the mechanism of endoplasmic MT generation in protonemal
cells of Physcomitrella patens. We developed an assay that utilizes flow cell and oblique
illumination fluorescence microscopy, which allowed visualization and quantification of …
studied using the cortical MT array, in which fixed-angled branching nucleation and katanin-
dependent MT severing predominate. However, little is known about MT generation in the
endoplasm. Here, we explored the mechanism of endoplasmic MT generation in protonemal
cells of Physcomitrella patens. We developed an assay that utilizes flow cell and oblique
illumination fluorescence microscopy, which allowed visualization and quantification of …
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