D2O-Isotope-Labeling Approach to Probing Phosphate-Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy
Increasing the bioavailability of immobilized phosphorus (P) in soil by phosphate-
solubilizing bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for
mitigating the P crisis. Here, D2O isotope labeling combined with single-cell Raman
spectroscopy (Raman–D2O) was developed as an efficient activity-based approach to
characterizing the presence and activity of PSB in a culture-independent way. On the basis
of the finding that PSB were significantly more active than non-PSB in the presence of …
solubilizing bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for
mitigating the P crisis. Here, D2O isotope labeling combined with single-cell Raman
spectroscopy (Raman–D2O) was developed as an efficient activity-based approach to
characterizing the presence and activity of PSB in a culture-independent way. On the basis
of the finding that PSB were significantly more active than non-PSB in the presence of …
D2O-isotope-labeling approach to probing phosphate-solubilizing bacteria in complex soil communities by single-cell Raman spectroscopy.
LHZ Li HongZhe, BQF Bi QingFang, YK Yang Kai… - 2019 - cabidigitallibrary.org
Increasing the bioavailability of immobilized phosphorus (P) in soil by phosphate-
solubilizing bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for
mitigating the P crisis. Here, D2O isotope labeling combined with single-cell Raman
spectroscopy (Raman-D2O) was developed as an efficient activity-based approach to
characterizing the presence and activity of PSB in a culture-independent way. On the basis
of the finding that PSB were significantly more active than non-PSB in the presence of …
solubilizing bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for
mitigating the P crisis. Here, D2O isotope labeling combined with single-cell Raman
spectroscopy (Raman-D2O) was developed as an efficient activity-based approach to
characterizing the presence and activity of PSB in a culture-independent way. On the basis
of the finding that PSB were significantly more active than non-PSB in the presence of …
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