Renal regeneration approaches offer great potential for the treatment of chronic kidney
disease, but their availability remains limited by the clinical challenges they pose. In the
present study, we used continuous detergent perfusion to generate decellularized (DC) rat
kidney scaffolds. The scaffolds retained intact vascular trees and overall architecture, along
with significant concentrations of various cytokines, but lost all cellular components. To
evaluate its potential in renal function recovery, DC scaffold tissue was grafted onto partially …

Introduction: For regeneration of complex organs, such as the kidney, decellularized (DC)
tissues are promising candidates that provide a neutral environment. Objective: We
successfully DC rhesus monkey kidneys by perfusion of trypsin/EDTA, sodium dodecyl
sulfate (SDS), triton X-100, and peracetic acid/ethanol. Subsequently, we analysed the
samples using quantitative and qualitative analysis. Methods: Histological staining, DNA
quantification and scanning electron microscopy (SEM) was used to investigate cellular …