This protocol describes how to order and directly assemble uracil-containing non-clonal
DNA fragments by uracil excision based cloning (USER cloning). The protocol was
generated with the goal of making synthesized non-clonal DNA fragments directly
compatible with USER TM cloning. The protocol is highly efficient and would be compatible
with uracil-containing non-clonal DNA fragments obtained from any synthesizing company.
The protocol drastically reduces time and handling between receiving the synthesized DNA …