Purpose Liquid biopsy using digital PCR (dPCR) has been widely used for the screening of
ESR1 mutations, since they are frequently identified in the hotspot. However, dPCR is
limited to the known mutations. Therefore, we aimed to analyze the utility of next-generation
sequencing (NGS) to discover novel ESR1 mutations. Methods Whole exon sequencing of
the ESR1 gene using NGS was performed in 16 primary and 47 recurrent tumor samples
and 38 plasma samples from hormone receptor-positive metastatic breast cancer patients …

CiNii 博士論文 - Detection of ESR1 mutations in plasma and tumors from metastatic breast
cancer patients using next-generation sequencing … Detection of ESR1 mutations in
plasma and tumors from metastatic breast cancer patients using next-generation
sequencing 転移性乳癌患者の血漿および腫瘍における次世代シーケンサーを用いたESR1変異の
検出 … Detection of ESR1 mutations in plasma and tumors from metastatic breast cancer
patients using next-generation sequencing …