Detection of neuronal growth inhibitory factor (metallothionein-3) in polyacrylamide gels and by Western blot analysis
G Meloni, M Knipp, M Vašák - Journal of biochemical and biophysical …, 2005 - Elsevier
Neuronal growth inhibitory factor (GIF) is a small cysteine-rich metal binding protein
downregulated in Alzheimer's disease. The protein belongs to the superfamily of
metallothioneins (MTs) and was classified as MT-3. Although first identified as a brain
specific protein, several reports now indicate a substantially broader expression pattern.
However, currently available detection methods for MT-3 show low sensitivity in gel
electrophoresis and Western blot. We have developed a fast and sensitive method for MT-3 …
downregulated in Alzheimer's disease. The protein belongs to the superfamily of
metallothioneins (MTs) and was classified as MT-3. Although first identified as a brain
specific protein, several reports now indicate a substantially broader expression pattern.
However, currently available detection methods for MT-3 show low sensitivity in gel
electrophoresis and Western blot. We have developed a fast and sensitive method for MT-3 …