Detection of starch granules in a living plant by optical second harmonic microscopy
G Mizutani, Y Sonoda, H Sano, M Sakamoto… - Journal of …, 2000 - Elsevier
G Mizutani, Y Sonoda, H Sano, M Sakamoto, T Takahashi, S Ushioda
Journal of Luminescence, 2000•ElsevierWe demonstrate a new method for detecting starch granules in a plant, based on optical
second harmonic microscopy. In this method a plant is irradiated with infrared laser pulses
and the image formed by scattered light at the second harmonic frequency is observed. A
starch granule consists of well-ordered arrays of chiral glucose molecules and shows an
intense optical second harmonic response. Thus they are selectively seen in the SH
intensity image.
second harmonic microscopy. In this method a plant is irradiated with infrared laser pulses
and the image formed by scattered light at the second harmonic frequency is observed. A
starch granule consists of well-ordered arrays of chiral glucose molecules and shows an
intense optical second harmonic response. Thus they are selectively seen in the SH
intensity image.
We demonstrate a new method for detecting starch granules in a plant, based on optical second harmonic microscopy. In this method a plant is irradiated with infrared laser pulses and the image formed by scattered light at the second harmonic frequency is observed. A starch granule consists of well-ordered arrays of chiral glucose molecules and shows an intense optical second harmonic response. Thus they are selectively seen in the SH intensity image.
Elsevier
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