Determination of cisapride in human plasma by high-performance liquid chromatography
MA Campanero, B Calahorra, E Garcia-Quetglas… - Chromatographia, 1998 - Springer
An HPLC method has been developed for the quantification of cisapride in plasma for
pharmacokinetic studies. Clebopride was used as internal standard. Plasma samples were
extracted at alkaline pH with tert-butyl methyl ether. The organic phase was then extracted
with sulphuric acid to eliminate endogenous interferences, and cisapride and the internal
standard were then extracted at alkaline pH into tert-butyl methyl ether. After evaporation of
tert-butyl methyl ether, the residue was analysed by HPLC. Chromatography was performed …
pharmacokinetic studies. Clebopride was used as internal standard. Plasma samples were
extracted at alkaline pH with tert-butyl methyl ether. The organic phase was then extracted
with sulphuric acid to eliminate endogenous interferences, and cisapride and the internal
standard were then extracted at alkaline pH into tert-butyl methyl ether. After evaporation of
tert-butyl methyl ether, the residue was analysed by HPLC. Chromatography was performed …
Determination of cisapride in human plasma by high-performance liquid chromatography with fluorescence detection
BD Kiss, KB Nemes, I Klebovich - Chromatographia, 2003 - Springer
Summary A new sensitive HPLC-FLD method has been developed and validated for the
determination of cisapride in human plasma for a bioequivalence study. A gradient method
was used to remove late-eluting plasma components of no interest. The separation was
performed on a Li-ChroCART 250-4 Purospher RP-18 (5 μm particle) analytical column
fitted with a LiChroCART 4-4 Purospher RP-18 endcapped (5 μm particle) guard column.
The excitation and emission wavelengths were 295 and 350 nm during fluorescence …
determination of cisapride in human plasma for a bioequivalence study. A gradient method
was used to remove late-eluting plasma components of no interest. The separation was
performed on a Li-ChroCART 250-4 Purospher RP-18 (5 μm particle) analytical column
fitted with a LiChroCART 4-4 Purospher RP-18 endcapped (5 μm particle) guard column.
The excitation and emission wavelengths were 295 and 350 nm during fluorescence …