Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles
R Kötitz, W Weitschies, L Trahms, W Brewer… - Journal of magnetism …, 1999 - Elsevier
R Kötitz, W Weitschies, L Trahms, W Brewer, W Semmler
Journal of magnetism and magnetic materials, 1999•ElsevierThe binding between avidin and biotinylated magnetic iron oxide nanoparticles can be
monitored by means of magnetic relaxation measurements. The resultant signal, caused by
particle aggregates, has Brownian and Néel components. The time constant and amplitude
of Brownian relaxation were determined by a fit algorithm which also yielded Néel
amplitudes proportional to the avidin content of the samples.
monitored by means of magnetic relaxation measurements. The resultant signal, caused by
particle aggregates, has Brownian and Néel components. The time constant and amplitude
of Brownian relaxation were determined by a fit algorithm which also yielded Néel
amplitudes proportional to the avidin content of the samples.
The binding between avidin and biotinylated magnetic iron oxide nanoparticles can be monitored by means of magnetic relaxation measurements. The resultant signal, caused by particle aggregates, has Brownian and Néel components. The time constant and amplitude of Brownian relaxation were determined by a fit algorithm which also yielded Néel amplitudes proportional to the avidin content of the samples.
Elsevier
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