Development and evaluation of double gene transgenic cotton lines expressing Cry toxins for protection against chewing insect pests
Cotton is the main fiber producing crop globally, with a significant impact on the economy of
Pakistan. Bt cotton expressing a Cry1Ac gene is grown over a large area in Pakistan,
however, there is a major concern that bollworms may develop resistance. Here we have
used a durable resistance strategy against bollworms by developing a double gene
construct containing Cry1Ac and Cry2Ab (pGA482-12R) for cotton transformation. Both Cry
toxin genes have been cloned in the same T-DNA borders and transferred successfully into …
Pakistan. Bt cotton expressing a Cry1Ac gene is grown over a large area in Pakistan,
however, there is a major concern that bollworms may develop resistance. Here we have
used a durable resistance strategy against bollworms by developing a double gene
construct containing Cry1Ac and Cry2Ab (pGA482-12R) for cotton transformation. Both Cry
toxin genes have been cloned in the same T-DNA borders and transferred successfully into …
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