Development and validation of duplex, triplex, and pentaplex real-time PCR screening assays for the detection of genetically modified organisms in food and feed

I Huber, A Block, D Sebah, F Debode… - Journal of Agricultural …, 2013 - ACS Publications
Worldwide, qualitative methods based on PCR are most commonly used as screening tools
for genetically modified material in food and feed. However, the increasing number and
diversity of genetically modified organisms (GMO) require effective methods for
simultaneously detecting several genetic elements marking the presence of transgenic
events. Herein we describe the development and validation of a pentaplex, as well as
complementary triplex and duplex real-time PCR assays, for the detection of the most …

[引用][C] Development and Validation of Duplex, Triplex, and Pentaplex Real-Time PCR Screening Assays for the Detection of Genetically Modified Organisms in Food …

H Ingrid, B Annette, S Daniela, D Frédéric, M Dany… - 2013
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