[PDF][PDF] Development of multiplex PCR assay for meat products authentication: Targeting double gene

MAM Hossain, SMK Uddin, SQ Bonny… - … Journal of Science …, 2019 - researchgate.net
MAM Hossain, SMK Uddin, SQ Bonny, MR Johan, ME Ali
Matter: International Journal of Science and Technology, 2019researchgate.net
… For the first time, here we targeted double gene sites in short-amplicon length multiplex
PCR (mPCR) for confirmed detection and differentiation of bovine, buffalo and porcine
materials in food chain. Multiple targets detection in single assay saves analytical cost and
time. The design of primer sets for mPCR … Here, we proposed the step by step
development of double genes targeted mPCR assay for the detection and differentiation of
beef, buffalo and pork simultaneously in a single assay platform. …
Abstract
Authentication of the species origins of animal-originated food products is a rapidly growing field because of its direct relevance to public health as well as people’s religious and cultural
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