To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy controls) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Overexpression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value= 0.032). The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.