Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-
MS) despite being increasingly used as a method for microbial identification, still present
limitations in which concerns the differentiation of closely related species. Bacillus pumillus
and Bacillus safensis, are species of biotechnological and pharmaceutical significance,
difficult to differentiate by conventional methodologies. In this study, using a well-
characterized collection of B. pumillus and B. safensis isolates, we demonstrated the …

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