Translocase mediates the transport of preproteins across the inner membrane of
Escherichia coli. SecA binds with high affinity to the membrane-embedded protein-
conducting SecYEG complex and serves as both a receptor for secretory proteins and as an
ATP-driven molecular motor. Cycles of ATP binding and hydrolysis by SecA drive the
progressive movement of the preprotein across the membrane. Surface plasmon resonance
allows an online monitoring of protein interactions. Here we report on the kinetic analysis of …