NK cells contribute to protective antitumor immunity, but little is known about the functional
states of NK cells in human solid tumors. To address this issue, we performed single-cell
RNA-seq analysis of NK cells isolated from human melanoma metastases, including lesions
from patients who had progressed following checkpoint blockade. This analysis identified
major differences in the transcriptional programs of tumor-infiltrating compared with
circulating NK cells. Tumor-infiltrating NK cells represented 7 clusters with distinct gene …