Dodeca satellite: a conserved G+ C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster.
JP Abad, M Carmena, S Baars… - Proceedings of the …, 1992 - National Acad Sciences
JP Abad, M Carmena, S Baars, RD Saunders, DM Glover, P Ludena, C Sentis, C Tyler-Smith…
Proceedings of the National Academy of Sciences, 1992•National Acad SciencesTo identify sequences from the centromeric region, we have constructed a Drosophila
melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA
from the minichromosome Dp (1; f) 1187 derived from the X chromosome. We describe the
structure of one clone isolated in this way. This YAC is structurally unstable and contains
tandemly repeated G+ C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most
of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences …
melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA
from the minichromosome Dp (1; f) 1187 derived from the X chromosome. We describe the
structure of one clone isolated in this way. This YAC is structurally unstable and contains
tandemly repeated G+ C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most
of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences …
To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.
National Acad Sciences
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