The application of bioglass coatings on metallic implants provides link between bone and materials and prevents corrosion of metallic implants in body fluid. Therefore, in this research, 45S5 bioglass-silica coatings on 316L stainless steel were prepared by the sol–gel method and were characterized by different techniques. According to X-ray diffraction (XRD) results, by sintering 45S5 bioglass at 600 °C for 5 h, coatings containing both amorphous phase and Na₂Ca₂Si₃O₉ crystalline phase were obtained. Scanning electron microscopy (SEM) results showed that coatings prepared via appropriate sol aging and substrate preparations are crack-free. Potentiodynamic polarization tests in simulated body fluid (SBF) showed that coatings improve corrosion resistance of substrates, significantly. Further, bioactivity was considered during 30 days of immersion in SBF at 37 °C by atomic absorption spectroscopy (AAS), XRD, SEM and potentiodynamic polarization tests that demonstrated the growth of amorphous apatite, hydroxyapatite (HA) and CaSiO₃ film on coatings.