Polyacrylonitrile (PAN) hollow fiber membranes were manufactured through the dry-jet wet spinning. Considering importance effect of structure of hollow fiber to separation performance, spinning parameters such as coagulants composition were changed to achieve suitable hollow fiber membranes. Water with different amount of glycerin was used as the bore liquid and coagulation bath. The effects of the glycerin concentration on the hollow fiber permeation and separation performance were investigated. Direct red 80 was used to measure the separation performance of hollow fiber module. Field emission scanning electron microscope (FE-SEM) was used to analyze the structure of the hollow fibers. The results proved that permeation is increased by adding glycerin to external and internal coagulation bath. In addition, hollow fibers spun in lower glycerin value would indicate lower hydraulic permeability, higher separation and higher tensile strength.